Chromatography is the process by which compounds inside a mixture are separated. This may be achieved by properties like size, and how the chemicals interact with the solid and mobile phases of chromatography. There are various kinds of chromatography, which range from 2D-based planar methods like Thin Layer Chromatography and column-based procedures, including Gas Chromatography and High-Performance Liquid Chromatography. Running the chromatography to produce a chromatogram involves using a mobile phase gas or liquid within a stationary phase liquid or solid, and also the way compounds interact with the two stages causes their separation. By way of instance, if a chemical binds strongly to the static phase then it will move slower than another molecule that doesn’t bind as strongly into the stationary phase.
In a planar chromatography technique, if a chemical binds more strongly into the static phase it travels up slower than chemicals which don’t bind as strongly. Consequently, the distance travelled by different substances in a mixture is observed in the chromatogram. In column chromatography, if a chemical binds more strongly into the static phase then this will be eluted from or leave the pillar after compounds which do not binds as strongly. These are also believed to have a longer retention period. Basic planar chromatography techniques may be used to identify if two ink samples will likely be the same. By way of instance, the ink used in two pieces of text may be compared to determine if they reveal a similar disturbance in planar chromatography.
As another example, a bank bill notice could be marked with ink, and if this bill is stolen then a forensic scientist might have the ability to see if the identical ink could be detected on the hands of the thief. Column what is chromatography method with a solid stationary phase and a liquid mobile phase. But, unlike liquid chromatography methods in which the mobile phase is slowly dripped through the column, it is pushed through the column at high pressure. HPLC can be used to test explosives as distinct materials used in explosives have different properties, and for that reason will have different retention times. Certain drugs may also be detected by HPLC.